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Of the mycobacteriophage L5 and a kanamycin resistant gene was used. The pimyc-ompATb fragment was removed from pML588 by XbaI and ClaI digestion, and ligated into similarly digested pCV125. The resulting plasmid was named pML759 (Table 2) and integrated into the L5 attB site of M. tuberculosis ML163 (ompATb::loxP). The complemented strain was named ML168 (ompATb::loxP, attB::pML759). All plasmid
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Of the mycobacteriophage L5 and a kanamycin resistant gene was used. The pimyc-ompATb fragment was removed from pML588 by XbaI and ClaI digestion, and ligated into similarly digested pCV125. The resulting plasmid was named pML759 (Table 2) and integrated into the L5 attB site of M. tuberculosis ML163 (ompATb::loxP). The complemented strain was named ML168 (ompATb::loxP, attB::pML759). All plasmid
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H reveals that the 5'-proximal region of the 5'-UTR has a cis-regulatory signature responsible for heat stress-regulated mRNA translation in Arabidopsis. Plant Cell Physiol. 54: 474-483. Mayberry, L.K., Allen, M.L., dennis, M.d., and Browning, K.S. (2009). Evidence for variation in the optimal translation initiation complex: plant eIF4B, eIF4F, and eIF(iso)4F differentially promote translation of
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