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Upplemental material). Interestingly, the signal in unstimulated HOIP / cells was lower than that in HOIP / cells, suggesting the presence of low levels of linear ubiquitination in resting cells, as was seen by immunoprecipitation (Fig. 4F; also, see Fig. S3F in the supplemental material). Using these observations, we defined linear-ubiquitinationpositive cells as cells with a fluorescence intensi
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47 A DOG’S LIFE (VIDA DE PERROS) Pocoyó quiere se un pato, un perro y un elefante, pero cuando Loula necesita su ayuda, nuestro amigo se da cuenta de que lo mejor es ser él mismo. 48 WHAT’S IN THE BOX? ¿QUÉ HAY EN LA CAJA? La nueva caja de Pocoyó cobra vida propia cuando Loula se mete debajo. Al final, con la ayuda de sus amigos, Pocoyó adivina QUIÉ
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Of interest. However, these two tracking approaches were unsuccessful and the expression of the exogenous kcnj212 protein could not be traced in injected zebrafish embryos.As the expression 95?8 kcnj2-12 and the increased expression of WT kcnj2-12 proteins have never been studied, embryos were injected with different amounts of RNA to determine an optimum amount for subsequent experiments. Embryos
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